Production Of Alkaline Protease From Poultry Waste

Abstract

Chicken egg shell and membrane were used as substrate for production of Alkaline protease by Bacillus. Bacillus species are attractive industrial microorganisms which have high growth rates leading to shorter fermentation times and possess the ability to secrete proteins. Bacterial fermentation using poultry egg processing waste, egg shell and membrane as substrate produces alkaline protease in significant level. Glucose as additional carbon source enhances enzyme production up to 13%. Additional of organic nitrogen sources like peptone and yeast extract increases enzyme production by 9% and 5%, respectively and inorganic nitrogen sources did not have any positive effect. The present study deals with the microbiological conversion of egg shell waste into valuable products like alkaline protease enzyme and its antimicrobial efficacy was analyzed against Staphylococcus aureus, Klebsiella pneumoniae, Escherichia coli strains.