Cardioprotection effects of diosgenin from Dioscorea bulbifera against isoproterenol-induced myocardial infarction

Abstract

This research plan is to examine the cardioprotective effects of bioactive diosgenin molecules isolated from Dioscorea plants on Isoproterenol (ISO)-induced myocardial infarction. Male albino Wistar rats were treated with D.bullifera and Diosgenin (20 mg/kg) daily for four weeks. After the experiment, the animals were injected with ISO (85 mg/kg) subcutaneously at 24 hour intervals for two days. The results describe the myocardial injury induced by ISO, which is manifested as an increase in serum cardiac marker enzymes CK-MB, C.K.P., G.O.T., and G.P.T. And L.D.H. and essential oxidative stress markers, such as LPO and G.S.H. In addition, significant increases in serum total cholesterol, triglycerides, and low-density lipoprotein cholesterol levels were also observed, while serum high-density lipoprotein cholesterol levels were reduced. Histopathological studies are also related to the above parameters. In addition to cardiac markers, lipid peroxidation levels in plasma and heart are also elevated. However, antioxidant enzymes such as glutathione peroxidase (G.P.X.), catalase, S.O.D. and reduced glutathione (G.S.T.) are significantly reduced in the heart after ISO-induced myocardial infarction. These findings summarized the cardioprotective effects of diosgenin and dioscorea on cardiac markers and antioxidant activity.