Isolation And Characterization Of Active Principle Hesperidin From Glycosmis Pentaphylla
Abstract
Medicinal plants are a pool of biologically active compounds with therapeutic properties that over time have been stated and used by diverse groups of people for treatment of various diseases. The present study aimed to isolate active principles from the ethanolic extract of plant Gycosmis pentaphylla. The isolation and structural characterization of bioactive compounds were carried out using various chromatographic techniques (TLC, column, HPLC, and GC-MS) and spectral studies such as IR, 1H-NMR, and 13C-NMR.Glycosmis pentaphylla belongs to Rutaceae family. It is commonly known as Orange berry (English). The plant has a long history of usage in traditional medicine against various illnesses around the world. In and other traditional medicinal practices the plant has been used against diseases like bilious complaints, cough, worms, jaundice, fever, inflammation, rheumatism, anaemia and vermifuge. . The plant also have some important pharmacological activities such as hepatoprotective, anti-inflammatory, anti-tumors, antioxidant, antibacterial, anti-viral, anti-ulcer, chemo protective and antiseptic properties were shown by researchers. The phytochemical assessment results show the presence of Pyrazole, cyclohexane, Flavone, pentadecane, Hesperidin, Methyl deconoic acid, elaidic acid Estra, diacetate, Octadecaenic acid, flavones, cyclohexanamine, Octadeconoic acid.Among them an important active constituent Hesperidine was isolated and characterized by chromatographic and spectral studies.