Lupoel, a triterpenoid possesses reno-protective effects via the suppression of NF-κB-mediated signaling in high fat diet and sucrose-fed diabetic nephropathy in Wistar rats

Abstract

Diabetic nephropathy (DN) is extremely severe and most general complication of diabetes which could be foremost reason of unceasing kidney failure. Aim: The aim of the present study was evaluated the reno-protective effect of lupeol in high fat diet and sucrose-fed diabetic nephropathy in Wistar rats. Methods: Lupeol was administered to the diabetic rats at a dose of (25 mg/kg body weight) was given once a day until the end of the study (60 days post induction of diabetes) to high fat diet-induced diabetic rats. Real Time-PCR analysis was performed in order to study reno-inflammatory signaling (IL-6, IL-1β and TNF-α). Results: Lupeol treatment significantly reduced hyperglycemia and significantly lowered oxidative stress markers in the renal tissue of treated diabetic groups. Moreover, lupeol treated animals exerted decrease in mRNA levels and low serum concentrations of IL-6, IL-1β and TNF-α compared to diabetic control rats. It showed a significant decrease in NF-kB activation in the diabetic kidney. Conclusion: Lupeol treatment mitigates oxidative stress and suppresses renal inflammation via inhibition of NF-kB in diabetic kidney in early progressive diabetic nephropathy. Therefore, the triterpenoid could be an effect treatment option in early progression of diabetic nephropathy in clinical trial.